RNA
Part:BBa_K3467004:Design
Designed by: Wang Ran Group: iGEM20_CAU_China (2020-10-21)
ATPase dsRNA sequence
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 823
Illegal PstI site found at 332 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 823
Illegal PstI site found at 332 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 823
Illegal BglII site found at 858 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 823
Illegal PstI site found at 332 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 823
Illegal PstI site found at 332 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Locust ATPase is partially identical to Human and other animals. When we designed the dsRNA sequence of ATPase, we should pay attention on the sequence identical rate.
Source
ATPase dsRNA sequence is from locust RNA extract. We use RT-PCR to get the cDNA of V-ATPase subunit H and attained ATPase dsRNA sequence by PCR with designed primers.